EC Number |
Protein Variants |
Reference |
---|
2.4.1.230 | D177N |
106% of wild-type activity |
-, 659537 |
2.4.1.230 | D271N |
69% of wild-type activity |
659537 |
2.4.1.230 | D340N |
89% of wild-type activity |
659537 |
2.4.1.230 | D362N |
complete loss of enzymic activity |
-, 659537 |
2.4.1.230 | D459N |
increase in Km-values for kojibiose, beta-D-glucose 1-phosphate and glucose |
659537 |
2.4.1.230 | D513N |
enhanced thermostablility and thermoactivity |
659532 |
2.4.1.230 | E284Q |
103% of wild-type activity |
659537 |
2.4.1.230 | E392R |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
736088 |
2.4.1.230 | E392R/T417A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
736088 |
2.4.1.230 | E392R/T417I |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
736088 |