   2.4.1.22 | more |
carbohydrate characterization of an antibody (Lo-BM2) expressed in Nicotiana tabacum strain BY-2 cells shows that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (about 70%) of the complex GnGnXF glycoform. The stable co-expression of a human beta-1,4-galactosyltransferase targeted to different Golgi subcompartments alters Lo-BM2N-glycosylation and results in the production of an antibody that exhibits either hybrid structures containing a low abundance of the plant epitopes (alpha-1,3-fucose and beta-1,2-xylose), or a large amount of galactose-extended N-glycan structures. Targeting of GalT to different Golgi subcompartments. The sequence corresponding to the catalytic region (amino acid residues 123-397) of the human GalT precursor is fused via a SpeI restriction site to the first 77 amino acids from Nicotiana tabacum GNTI, corresponding to the CTS domain |
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