   2.4.1.216 | more |
endogenous genes trePP and pgmB, encoding trehalose 6-phosphate phosphorylase and beta-phosphoglucomutase, respectively, provide the synthesis of trehalose 6-phosphate, which can be transformed into trehalose by transgenically expressed trehalose 6-phosphate phosphatase, the respective gene otsB from the food-grade organism Propionibacterium freudenreichii is expressed in Lactobacillus lactis. Trehalose-producing strains show improved tolerance of 5 to 10fold higher survivability to acid at pH 3.0 and cold shock of 4°C, also a strong improvement in cell survival in response to heat shock of 45°C is achieved, but no protection is rendered against dehydration, phenotype overview |
-, 721344 |
