EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.211 | C236E |
random mutagenesis, the mutant shows decreased thermostability and reduced activity compared to the wild-type |
737186 |
| 2.4.1.211 | C236F |
random mutagenesis, the mutant shows highly increased thermostability and inacreased activity compared to the wild-type |
737186 |
| 2.4.1.211 | C236H |
random mutagenesis, the mutant shows slightly increased thermostability compared to the wild-type |
737186 |
| 2.4.1.211 | C236P |
random mutagenesis, the mutant shows highly increased thermostability and reduced activity compared to the wild-type |
737186 |
| 2.4.1.211 | C236W |
random mutagenesis, the mutant shows slightly increased thermostability compared to the wild-type |
737186 |
| 2.4.1.211 | C236Y |
random mutagenesis, the mutant shows highly increased thermostability and increased activity compared to the wild-type. In the mutant, the hydroxyl group of Tyr236 forms a hydrogen bond with the carboxyl group of E319. Mutant C236Y shows a 1.6fold higher specific activity than the wild-type |
-, 737186 |
| 2.4.1.211 | C236Y/D576V |
random mutagenesis, the mutant shows highly increased thermostability compared to the wild-type |
737186 |
| 2.4.1.211 | D108N |
site-directed mutagenesis |
685657 |
| 2.4.1.211 | D160N |
site-directed mutagenesis |
685657 |
| 2.4.1.211 | D182N |
site-directed mutagenesis |
685657 |
