EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.14 | D498A |
the mutant shows strongly decreased activity compared to the wild type enzyme |
756201 |
| 2.4.1.14 | more |
construction of multiple knockout mutants of the four Arabidopsis sucrose phosphate synthase isozymes SPSA1, SPSA2, SPSB, and SPSC (T-DNA insertion mutants spsa1 (SALK 119162), spsa2 (SALK 064922), spsb (GABI 368F01) and spsc (SAIL 31 H05) and mutants spsa1/spsa2, spsa1/spsb, spsa1/spsc, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsb/spsc, spsa1/spsa2/spsb, spsa1/spsa2/spsc, spsa2/spsb/spsc, and spsa1/spsa2/spsb/spsc). Despite their reduced SPS activity, mutants spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc display wild-type vegetative and reproductive morphology, and show wild-type photosynthetic capacity and respiration, phenotypes overview. Growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants is reduced compared with wild-type plants. The plants display a high dark respiration phenotype. Mutant spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinate and produce aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulate wild-type levels of nonstructural carbohydrates. Mutant spsa1/spsc leaves possess high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulate high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions |
737045 |
| 2.4.1.14 | more |
construction of N-terminally truncated enzymeslacking up to 171 amino acid residues in the 20 kDa N-terminal region, the mutants cannot be activated |
736380 |
| 2.4.1.14 | more |
enzyme downregulation by gene SPS1 antisense expression in transgenic plants using the CMV 35S promoter and Agrobacterium tumefaciens LBA4404 transfection. The transgenic plants show a phenotype with reduces sucrose phosphate synthase activity, fruit size and sucrose concentration, affected muskmelon leaf subcellular structure in SPS1 antisense muskmelon, overview |
702098 |
| 2.4.1.14 | more |
expression of the SPS gene from Arabidosis thaliana in Populus alba x Populus grandidentata causes an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview |
706865 |
| 2.4.1.14 | more |
overexpression of the enzyme from Zea mays in Medicago sativa, gene SPS-1 or SPSA expression is driven by the constitutive CaMV35S promoter. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions, phenotype, overview |
737054 |
| 2.4.1.14 | more |
production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview |
737053 |
| 2.4.1.14 | P497A |
the mutant shows slightly increased activity compared to the wild type enzyme |
756201 |
| 2.4.1.14 | R496A |
the mutation leads to loss of enzyme activity |
756201 |
| 2.4.1.14 | S495A |
the mutant shows about 2fold increased activity compared to the wild type enzyme |
756201 |
