EC Number   |
Protein Variants |
Reference |
|---|
    2.4.1.133 | A186D |
mutant shows decreased enzymatic activity compared to wild-type, Km towards donor and acceptor substrate increased compared to wild-type |
718777 |
| 2.4.1.133 | A186D |
the mutant exhibits 9times reduced activity compared to the wild type enzyme |
722265 |
| 2.4.1.133 | A186D |
the mutation is associated with the progeroid form of the Ehlers-Danlos syndrome and with the reduction of enzyme activity. The mutation weakly impairs the binding of the donor substrate. Glycosaminoglycan biosynthesis is slightly affected by the mutation |
718777 |
| 2.4.1.133 | D163A |
inactive |
719897 |
| 2.4.1.133 | D165A |
inactive |
719897 |
| 2.4.1.133 | D165E |
the apparent Km values of the D165E mutant toward UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside are about 3.8 and 5.5fold, respectively, higher than the wild type enzyme |
719897 |
| 2.4.1.133 | D211N |
inactive |
736498 |
| 2.4.1.133 | D211N |
mutant with negligible catalytic activity |
736433 |
| 2.4.1.133 | D211N |
site-directed mutagenesis, inactive mutant |
736433 |
| 2.4.1.133 | D211N |
site-directed mutagenesis, the catalytic base Asp211 is mutated to Asn residue, inactive mutant, substrate-bound crystal structure analysis |
736433 |
