EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.8 | C159A |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | C159A/C277A/C312A/C325A |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | C159S |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | C277A |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | C277A/C312A/C325A |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | C312A |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | C325A |
Km similar to wild-type enzyme |
487542 |
| 2.3.1.8 | D309A |
complete loss of activity, mutation does not affect the dimerization |
757471 |
| 2.3.1.8 | D316E |
kcat for the reaction of acetyl phosphate and CoA is 2.4fold lower than wild-type value, Km for CoA is 1.1fold higher than wild-type value, Km for acetyl phosphate is 1.4fold lower than wild-type value |
674262 |
| 2.3.1.8 | D318A |
complete loss of activity, mutant displays a broad biphasic pattern in gel filtration |
-, 757471 |
