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EC Number
Protein Variants
Commentary
Reference
2.3.1.74
A133S
fully functional enzyme
486402
2.3.1.74
C135A
77% enzyme activity
487455
2.3.1.74
C164A
reduced activity
487476
2.3.1.74
C164S
inhibition by diethyl diphosphate is pH dependent
487481
2.3.1.74
C169A
no enzyme activity
487455
2.3.1.74
C169S
no enzyme activity
487482
2.3.1.74
C169S/Q100E
28% of wild-type activity
487482
2.3.1.74
C169S/Q100E/K180Q
15% of wild-type activity
487482
2.3.1.74
C170R
more than 50% decrease in activity with hexanoyl-CoA, increase in thermal stability
719618
2.3.1.74
C170S
more than 80% decrease in activity with hexanoyl-CoA, increase in thermal stability
719618
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