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Results 1 - 10 of 27 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4biotechnology engineering of a metabolic pathway for high level production of N-acetylglucosamine in Escherichia coli by overexpressing Escherichia coli glucosamine synthase (GlmS) and Saccharomyces cerevisiae glucosamine-6-phosphate acetyltransferase (GNA1) 673392
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4C157A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity 755991
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4E156A Km: 1.2 mM (substrate: glucosamine 6-phosphate), kcat = 9.7/sec (substrate: glucosamine 6-phosphate) 686767
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4E156D Km: 0.175 mM (substrate: glucosamine 6-phosphate), kcat = 19.3/sec (substrate: glucosamine 6-phosphate) 686767
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4E185Y Km (mM): 0.03 (D-glucosamine 6-phosphate), 0.04 (acetyl-CoA), kcat (1/sec): 1.34 (D-glucosamine 6-phosphate) 685012
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4G183E Km (mM): 0.056 (D-glucosamine 6-phosphate), 0.1 (acetyl-CoA), kcat (1/sec): 0.34 (D-glucosamine 6-phosphate) 685012
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4G68S the mutant called lignescens, a temperature-sensitive mutant that exhibits ectopic lignin deposition and growth defects under high-temperature conditions, is due to single base transition G68S in glucosamine-6-phosphate N-acetyltransferase. When exposed to the restrictive temperature, the mutant strain contains a significantly smaller amount of UDP-GlcNAc than the wild type. The growth defects and ectopic lignification of the mutant are suppressed by the addition of UDP-GlcNAc. N-glycans are reduced and luminal binding protein 3, a typical UPR gene, is expressed in the mutant strain at the restrictive temperature. Treatment with UPR-inducing reagents phenocopies the mutant 720693
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4L138A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 1 755991
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4L155A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity 755991
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.4L155A/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity 755991
Results 1 - 10 of 27 > >>