EC Number |
Protein Variants |
Reference |
---|
2.3.1.255 | E24A |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |
2.3.1.255 | E24D |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |
2.3.1.255 | E24Q |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |
2.3.1.255 | E61A |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |
2.3.1.255 | E62A |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |
2.3.1.255 | F128I |
site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type |
756675 |
2.3.1.255 | F128L |
site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type |
756675 |
2.3.1.255 | H111A |
mutation in NatA, decrease in kcat, Km value similar to wild-type |
736866 |
2.3.1.255 | H20A |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |
2.3.1.255 | H72A |
mutation in NatA, decrease in kcat, increase in Km value |
736866 |