EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.17 | C128A |
mutation in region 4, 94% of wild-type expression, 88% of wild-type activity |
718791 |
| 2.3.1.17 | C139A |
mutation in region 4, 70% of wild-type expression, 126% of wild-type activity |
718791 |
| 2.3.1.17 | D168A |
mutation in region 5, 46% of wild-type expression, no residual activity |
718791 |
| 2.3.1.17 | D168E |
mutation in region 5, 63% of wild-type expression, 7% of wild-type activity |
718791 |
| 2.3.1.17 | E101A |
mutation in region 3, 97% of wild-type expression, 42% of wild-type activity |
718791 |
| 2.3.1.17 | E101D |
mutation in region 3, 61% of wild-type expression, 99% of wild-type activity |
718791 |
| 2.3.1.17 | more |
transfection of truncated forms of isoform NAT8L into HEK-293T cells indicates that the 68 N-terminal residues, i.e. regions 1 and 2, have no importance for the catalytic activity and the subcellular localization of the enzyme. The membrane region, i.e. region 4, is necessary and sufficient to target isoform NAT8L to the endoplasmic reticulum |
718791 |
| 2.3.1.17 | P142A |
mutation in region 4, 113% of wild-type expression, 116% of wild-type activity |
718791 |
| 2.3.1.17 | R133A |
mutation in region 4, 78% of wild-type expression, 64% of wild-type activity |
718791 |
| 2.3.1.17 | R133K |
mutation in region 4, 69% of wild-type expression, 89% of wild-type activity |
718791 |
