EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.110 | F145Y |
KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 3fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin |
676598 |
| 2.3.1.110 | F145Y/Y147F |
KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 4.2fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin |
676598 |
| 2.3.1.110 | more |
a series of chimeric genes is constructed by reciprocal exchange of DNA segments between the serotonin N-hydroxycinnamoyltransferase and tyramine N-hydroxycinnamoyltransferase cDNAs. Functional characterization of the recombinant chimeric proteins revealed that the amino acid residues 129 to 165 of serotonin N-hydroxycinnamoyltransferase and the corresponding residues 125 to 160 in tyramine N-hydroxycinnamoyltransferase are critical structural determinants for amine substrate specificity |
676598 |
| 2.3.1.110 | more |
fusion construct of THT and tyrosine decarboxylase |
702831 |
