EC Number |
Protein Variants |
Reference |
---|
2.1.4.1 | F245N |
site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT. F245N variant seems to lack the potential ligand-induced conformational changes. Higher activity of the F245N variant is enthalpy-driven |
-, 719473 |
2.1.4.1 | F245N/S247M |
site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT |
-, 719473 |
2.1.4.1 | medicine |
in vitro assay for enzyme activity in lymphocytes based on stable-isotope-labeled substrates L-(guanidino-15N2)arginine and U-(13C,15N)glycine |
661505 |
2.1.4.1 | more |
the residue replacements do not change the kinetic mechanism of the three variant enzymes. The mutant protein variants have broad substrate specificity, e.g. the F245N variant additionally accepts creatine, with 7% relative activity |
-, 719473 |
2.1.4.1 | S247M |
site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT |
-, 719473 |