EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.300 | E205A |
site-directed mutagenesis, replacement of the residue with Ala results in a small decrease in protein stability as observed through a slight reduction in melting temperature, and to a large decrease in activity for three different substrates, especially the best substrate identified so far, (S)-reticuline |
757162 |
| 2.1.1.300 | E205A/H206A |
site-directed mutagenesis, replacement of Glu205 and His206 with Ala leads to large decreases in activity for three different substrates to about 10% of wild-type activity at pH 7.5 |
757162 |
| 2.1.1.300 | E80A |
site-directed mutagenesis, the mutant shows 2fold increased activity compared to wild-type with substrate (S)-reticuline |
757162 |
| 2.1.1.300 | H206A |
site-directed mutagenesis, substrate binding structures compared to the wild-type enzyme, overview. Replacement of the residue with Ala results in a small decrease in protein stability as observed through a slight reduction in melting temperature, and to a large decrease in activity for three different substrates, especially the best substrate identified so far, (S)-reticuline |
757162 |
| 2.1.1.300 | Y79A |
site-directed mutagenesis, inactive mutant |
757162 |
