EC Number |
Protein Variants |
Reference |
---|
2.1.1.180 | D21A |
the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme |
734807 |
2.1.1.180 | D30A |
mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function SAM-binding: mutant highly affected |
720586 |
2.1.1.180 | D36A |
inactive |
734807 |
2.1.1.180 | D55A |
mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function SAM-binding: mutant highly affected |
720586 |
2.1.1.180 | D61A |
inactive |
734807 |
2.1.1.180 | E146A |
functionally compromised mutant |
-, 735187 |
2.1.1.180 | E184C |
introduction of a residue displaying high modification efficiency with other Cys-reactive reagents for fluorescence assays. Mutant binds to 30S and dissociates upon addition of SAM |
755802 |
2.1.1.180 | E188C |
introduction of a residue displaying high modification efficiency with other Cys-reactive reagents for fluorescence assays. Mutant binds to 30S but fails to dissociate upon addition of SAM |
755802 |
2.1.1.180 | E88A |
mutant protein is tested for its ability to support bacterial growth in the presence of kanamycin. Proposed function RNA/30S binding: mutant moderately affected |
720586 |
2.1.1.180 | E94A |
the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme |
734807 |