EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.171 | D58A |
site-directed mutagenesis, the mutant shows increased Km toward the 30S subunit by two orders of magnitude with only a marginal effect on kcat compared to the wild-type enzyme. The mutation decreases the enthalpic contribution to SAM binding, paralleled by an increase in the entropic contribution |
721033 |
| 2.1.1.171 | D58A/P128A/P129A |
site-directed mutagenesis, the mutant shows increased Km toward the 30S subunit by two orders of magnitude with only a marginal effect on kcat compared to the wild-type enzyme |
721033 |
| 2.1.1.171 | F130S |
site-directed mutagenesis |
721033 |
| 2.1.1.171 | more |
knockout of the rsmD gene and complementation by point mutants, except for mutant P128A/P129A/F130S, overview |
721033 |
| 2.1.1.171 | P128A/P129A |
site-directed mutagenesis, the mutation decreases the enthalpic contribution to SAM binding, paralleled by an increase in the entropic contribution, |
721033 |
| 2.1.1.171 | P128A/P129A/F130S |
site-directed mutagenesis, inactive mutant |
721033 |
