EC Number |
Protein Variants |
Reference |
---|
2.1.1.140 | E204A |
4-6% of wild-type activity |
755791 |
2.1.1.140 | E204G |
mutation to the corresponding residue of Papaver somniferum reticuline N-methyltransferase. Mutant shows a weak activity with (S)-coclaurine and a more substantial activity with (S)-N-methylcoclaurine. Compared to wild-type, activity with (S)-reticuline is 35fold improved |
757949 |
2.1.1.140 | E207A |
60-75% of wild-type activity |
755791 |
2.1.1.140 | F332A |
0.6% of wild-typ activity with substrate coclaurine |
755791 |
2.1.1.140 | G331A |
negligible effect on activity |
755791 |
2.1.1.140 | H208A |
1% of wild-type activity with substrate heliamine, 4% with substrate coclaurine, respectively |
755791 |
2.1.1.140 | more |
virus-induced gene silencing in opium poppy to functionally characterize six genes potentially involved in papaverine biosynthesis, e.g. the gene encoding coclaurine N-methyltransferase, altered alkaloid content phenotypes, overview. Norlaudanine is only detected in CNMT-suppressed plants |
720710 |
2.1.1.140 | R330A |
negligible effect on activity |
755791 |
2.1.1.140 | W329A |
4% of wild-type activity with substrate heliamine, 0.5% with substrate coclaurine, respectively |
755791 |
2.1.1.140 | Y328A |
5% of wild-type activity with substrate heliamine, 10% with substrate coclaurine, respectively |
755791 |