EC Number   |
Protein Variants |
Reference |
|---|
    1.5.3.17 | D94N |
site-directed mutagenesis, the mutation primarily affects the reductive half-reaction. The mutant shows 20-40fold reduced rate constant for flavin reduction with spermine and 450fold with N1-acetylspermine compared to the wild-type enzyme |
724359 |
| 1.5.3.17 | H64A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
724126 |
| 1.5.3.17 | H64E |
Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym |
742614 |
| 1.5.3.17 | H64N |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
724126 |
| 1.5.3.17 | H64Q |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
724126 |
| 1.5.3.17 | H64Q |
Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym |
742614 |
| 1.5.3.17 | H67A |
site-directed mutagenesis, the mutant shows a 2-3fold reduced first-order rate constant for flavin reduction and slightly altered kinetics compared to the wild-type enzyme |
724344 |
| 1.5.3.17 | H67N |
site-directed mutagenesis, the mutant shows a 2-3fold reduced first-order rate constant for flavin reduction and slightly altered kinetics compared to the wild-type enzyme |
724344 |
| 1.5.3.17 | H67Q |
site-directed mutagenesis, the mutant shows a 2-3fold reduced first-order rate constant for flavin reduction and slightly altered kinetics compared to the wild-type enzyme |
724344 |
| 1.5.3.17 | H69E |
compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine |
742614 |
