EC Number   |
Protein Variants |
Reference |
|---|
    1.5.3.16 | E216L/S218A |
mutation E216L/S218A endows the enzyme with N1-acetylspermine oxidase activity |
741851 |
| 1.5.3.16 | H82E |
no catalytic activity |
710832 |
| 1.5.3.16 | H82E |
site-directed mutagenesis, inactive mutant |
710832 |
| 1.5.3.16 | H82Q |
about 300fold decrease in catalytic efficiency |
710832 |
| 1.5.3.16 | H82Q |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
710832 |
| 1.5.3.16 | K367M |
about 3fold decrease in catalytic efficiency |
710832 |
| 1.5.3.16 | K367M |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
710832 |
| 1.5.3.16 | more |
a mutant enzyme deprived of all but two cysteine residues (C263/C429) results in a monomeric enzyme species. The mutant enzyme is less stable than the wild type. Is activity decreased by about 90% after 1 day from the preparation, and storage at 6°C or -20°C |
741586 |
| 1.5.3.16 | more |
construction of a knock-out mutant that has a T-DNA insertion within the ninth exon of the AtPAO4 gene, and of a knock-down mutant Atpao4i with reduced expression by RNAi under control of the CMV 35S promoter. AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis |
694651 |
| 1.5.3.16 | more |
purified mutant mSMOmuDELTA (with a deletion of the nuclear domain A) exhibits biochemical properties very similar to that of mSMOalpha and mSMOmu, in particular a pH optimum of 8.5 in 0.1 m Na-phosphate buffer, a Km value of 0.222 mM and a kcat value of 0.0208 per sec. Removal of the nuclear domain amino acid region abolishes proper nuclear targeting of the mSMOmu isoform |
692280 |
