EC Number |
Protein Variants |
Reference |
---|
1.5.1.30 | E99K |
the mutation destabilizes the dimer and has an enhanced subunit dissociation as evident from a 44fold higher Kd for the monomer-dimer equilibrium |
684711 |
1.5.1.30 | K167A |
the mutant has apparently greatly increased Km and reduced kcat/Km for NADPH |
724343 |
1.5.1.30 | L114M/L165M |
mutant created to obtain selenomethionine-containing enzyme for crystallization. Activity of mutant is similar to wild-type, circular dichroism spectra identical to wiil-type |
671139 |
1.5.1.30 | more |
Fre oxidoreductase deletion strain shows 99% reduced bioluminescence in coupled assay with luciferase |
698992 |
1.5.1.30 | more |
generation of a WU2DELTAflaRkan null mutant strain |
765814 |
1.5.1.30 | more |
improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Construction of mutants of gene frd2, strain 5-5(frd2) and deletion strains 5-5DELTAfrd2 and 5-5DELTAfrd12. The extracellular H2O2 concentrations of mutant 5-5DELTAfrd12 are lower than that of the wild-type strain SYPA5-5, and the extracellular H2O2 concentrations of mutant 5-5(frd2) is increased compared to the wild-type. Flavin reductase activities in frd1 and frd2 overexpression and deletion strains with NADH and FAD, overview |
-, 743224 |
1.5.1.30 | N134A |
the mutant shows increased Km and reduced kcat/Km for NADPH |
724343 |
1.5.1.30 | Q14R |
mutation at active site arginine residue increases coenzyme affinity |
765021 |
1.5.1.30 | Q14R/R78G |
double mutation within the enzyme exhibits a binding affinity that is close to the average between these two individual mutations |
765021 |
1.5.1.30 | R133A |
the mutant shows increased Km and reduced kcat/Km for NADPH |
724343 |