EC Number   |
Protein Variants |
Reference |
|---|
   1.3.7.7 | C112A |
inactive |
-, 713102 |
| 1.3.7.7 | C26A |
inactive |
-, 713102 |
| 1.3.7.7 | C51A |
the mutation almost abolishes the activity of the enzyme (less than 5% compared to the wild type enzyme) |
-, 713102 |
| 1.3.7.7 | C95A |
inactive |
713102 |
| 1.3.7.7 | C95A |
inactive protein, probably due to destabilization of the [4Fe-4S] cluster environment |
712465 |
| 1.3.7.7 | C95S |
inactive protein, probably due to destabilization of the [4Fe-4S] cluster environment |
712465 |
| 1.3.7.7 | D36A |
site-directed mutagenesis, mutation in BchB, mutant NB-cluster structure compered to the wild-type enzyme |
-, 713102 |
| 1.3.7.7 | D36A |
site-directed mutagenesis, the mutant subunit B forms a complex with subunit N, indicating that Asp36 is not necessary for complex formation, D36A retains only 13% of wild-type activity |
726526 |
| 1.3.7.7 | D36A |
the mutant exhibits low activity (13% compared to the wild type enzyme) |
-, 713102 |
| 1.3.7.7 | D36C |
catalytically inactive |
712026 |
