EC Number   |
Protein Variants |
Reference |
|---|
   1.3.1.97 | more |
generation of an expression system for production of high levels of botryococcene in Nicotiana tabacum strain KY 1068 plants by diverting carbon flux from the cytosolic mevalonate pathway or the plastidic methylerythritol phosphate pathway by the targeted overexpression of an avian farnesyl diphosphate synthase along with botryococcene synthase SSL-3 and presqualene diphosphate synthase SSL-1, overview. The structure of purified botryococcene from tobacco is determined by 1H-NMR and 13C-NMR spectral analyses. Modifications of botryococcene in tobacco by coexpressed triterpene methyltransferases (TMTs) from Botryococcus braunii |
746113 |
| 1.3.1.97 | N171A/G207Q |
site-directed mutagenesis, combined N171A and G207Q mutations in the SSL-3 backbone result in complete conversion of SSL-3 from an enzyme that used presqualene diphosphate for botryococcene biosynthesis to one that used presqualene diphosphate for squalene biosynthesis |
741903 |
| 1.3.1.97 | Y166A |
site-directed mutagenesis, mutant substrate specificity and activity compared to the wild-type |
741903 |
| 1.3.1.97 | Y166F |
site-directed mutagenesis, mutant substrate specificity and activity compared to the wild-type |
741903 |
