   1.3.1.71 | more |
analysis of sterol compositions of wild-type Sacchromyces cerevisiae strain BY4742, the erg6 knockout mutant (DELTAerg6), and DELTAerg6 expressing the Pneumocystis carinii or the Saccharomyces cerevisiae erg6 gene by a variety of chromatographic and spectroscopic procedures. The Pneumocystis carinii SAM:SMT in the DELTAerg6 restores its ability to produce the C28 sterol ergosterol as the major sterol, and also results in low levels of C29 sterols, overview. While the Pneumocystis carinii SAM:SMT in the yeast DELTAerg6 cells is able to transfer a second methyl group to the side chain, the action of DELTA24(28)-sterol reductase (coded by the erg4 gene) in the yeast cells prevents the formation and accumulation of as many C29 sterols as that found in Pneumocystis carinii |
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