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EC Number
Protein Variants
Commentary
Reference
1.3.1.20
A36D
production by site-directed mutagenesis
684150
1.3.1.20
A36D
shows a decreased kcat value compared to the wild type enzyme
684150
1.3.1.20
C145S
same sensitivity to SH-reagents as wild-type
349250
1.3.1.20
C170A
kinetically similar to wild-type
349257
1.3.1.20
C193S
no sensitivity to SH-reagents remaining
349250
1.3.1.20
C217A
4fold increase in Km for 5alpha-androstan-3,17-dione and 2fold increase in Km for NADH
349257
1.3.1.20
C87S
DD1 mutant
349261
1.3.1.20
D280A
production by site-directed mutagenesis
684150
1.3.1.20
D280A
shows a decreased kcat value compared to the wild type enzyme
684150
1.3.1.20
D50N
low dehydrogenase activity
349250
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