EC Number   |
Protein Variants |
Reference |
|---|
   1.21.3.1 | C104S |
single-strand-site-directed mutagenesis, loss of more than 96% activity |
440321 |
| 1.21.3.1 | C106S |
site-directed mutagenesis, 63% reduced activity, 14fold increased Km for N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine |
440317 |
| 1.21.3.1 | C106S/C255S |
site-directed mutagenesis, 63% reduced activity, 14fold increased Km for N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine |
440317 |
| 1.21.3.1 | C142S |
single-strand-site-directed mutagenesis, loss of 24% activity |
440321 |
| 1.21.3.1 | C251S |
single-strand-site-directed mutagenesis, loss of 47.7% activity |
440321 |
| 1.21.3.1 | C255S |
site-directed mutagenesis, 33% reduced activity, 1.4fold increased Km for N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine |
440317 |
| 1.21.3.1 | C37S |
single-strand-site-directed mutagenesis, loss of 18.3% activity |
440321 |
| 1.21.3.1 | C37S/C142S/C251S |
triple mutant, conformationally different from wild-type, prepared by recombining fragments of IPNS-encoding gene pcbC from each of the single mutants, loss of more than 90% activity |
440321 |
| 1.21.3.1 | D214C |
site-directed mutagenesis, active site mutant, complete loss of activity |
440328 |
| 1.21.3.1 | D214E |
site-directed mutagenesis, active site mutant, retains 1% of activity compared to wild-type |
440328 |
