EC Number |
Protein Variants |
Reference |
---|
1.20.99.1 | C120A |
inactive mutant |
714217 |
1.20.99.1 | C71S |
mutagenesis of cysteine residues in the putative active site HC(X) 5 R motif leads to nearly complete loss of both phosphatase and arsenate reductase activities |
694407 |
1.20.99.1 | more |
catalytic domain of Cdc25B is expressed in bacteria. Cdc25B shows arsenate reductase activity. Enzyme reduces inorganic arsenate but not methylated pentavalent arsenicals. Alteration of either the cysteine and arginine residues of the Cys-X5-Arg motif leads to the loss of both reductase and phosphatase activities |
714217 |
1.20.99.1 | R126A |
inactive mutant |
714217 |
1.20.99.1 | R60A |
site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity |
660461 |
1.20.99.1 | R60K |
site-directed muatgenesis, crystal structure determination and analysis with bound arsenite, formation of a covalent and more stableCys12-thiol-dihydroxyarsenite intermediate compared to the wild-type enzyme, resulting in slow release of product and loss of activity |
660461 |