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Results 1 - 3 of 3
EC Number Protein Variants Commentary Reference
Show all pathways known for 1.2.1.87Display the reaction diagram Show all sequences 1.2.1.87I195F created by site-specific mutagenesis, no significant reduction of channeling efficiency of the enzyme complex toward acetaldehyde or propionaldehyde 721651
Show all pathways known for 1.2.1.87Display the reaction diagram Show all sequences 1.2.1.87I195L created by site-specific mutagenesis 721651
Show all pathways known for 1.2.1.87Display the reaction diagram Show all sequences 1.2.1.87more design of a coenzyme A (CoA) dependent n-butanol biosynthesis pathway tailored to the metabolic physiology of the cyanobacterium Synechococcus elongatus PCC 7942 by incorporating an ATP driving force and a kinetically irreversible trap. Oxygen-sensitive CoA-acylating butyraldehyde dehydrogenase (Bldh) is exchanged for the oxygen-tolerant PduP from Salmonella enterica. Replacing Bldh with PduP in the n-butanol synthesis pathway results in n-butanol production to a cumulative titer of 404 mg/l with peak productivity of 51 mg/l per day, exceeding the base strain by 20fold. Anaerobic growth rescue of Escherichia coli strain JCL166 by overexpression of the Clostridium butanol pathway with different aldehyde dehydrogenases PduP 742372
Results 1 - 3 of 3