EC Number   |
Protein Variants |
Reference |
|---|
   1.14.18.4 | F49S/V242A/H319Q |
naturally occuring mutation in the OLE-1 mutant strain i.e. high-oleic castor mutant. Residue F49 is located in domain I, residue V242 in transmembrane domain TM3, and residue H319 in the His box III or domain IX, respectively, In the high-oleic mutant seed, embryos mostly accumulate palmitic, oleic and linoleic in the first stages of development, which is followed by an important accumulation of oleic acid during seed maturation. The ricinoleic acid content remains low in these seeds throughout development, in contrast to the wild-type seeds. Comparison of fatty acid profiles of seeds. The mutant displays a phenotype similar to the control cells that are transformed with the empty pYES2 plasmid. Structural effect of the mutations, overview |
-, 746157 |
| 1.14.18.4 | M295L |
site-directed mutagenesis, expression of the mutant FAH12gene in yeast does not result in any obvious changes in the ratio of hydroxy and diunsaturated fatty acids accumulating |
689659 |
| 1.14.18.4 | more |
fusion enzyme of castor bean oleate 12-hydroxylase and tobacco cytochrome b5 |
697014 |
| 1.14.18.4 | more |
optimization of the recombinant enzyme from gene FAH12 overexpression in Schizosaccharomyces pombe, overview. The enzyme product is toxic for the yeast cells. Gene plg7, encoding phospholipase A2, acts as a multicopy suppressor restoring the growth defect by removing ricinoleic acid from phospholipids and inducing secretion of a part of the released free ricinoleic acid into culture media. Overexpression of Schizosaccharomyces pombe triglyceride (TG) lipase encoded by gene ptl2, but not the expression of the TG lipases encodd by ptl1 and ptl3, suppresses the growth defect induced by ricinoleic acid production, and the culture grown at 20°C secretes free ricinoleic acid into media like plg7 overexpression. Suppression by ptl2 is independent of plg7, and a large amount of free ricinoleic acid is accumulated in the cells concomitant with the decrease in ricinoleic acid moieties in phospholipids. The suppression by ptl2 is attenuated by bromoenol lactone (BEL), a phospholipase A2 specific inhibitor, suggesting that Ptl2p may have phospholipase activity. Simultaneous overexpression of ptl2 and plg7 in the FAH12 integrant increases secretion and intracellular accumulation of ricinoleic acid 1.2 and 1.3fold, respectively, compared to those with single overexpression of plg7 on day 10 at 20°C. Method optimization and evaluation, overview |
744145 |
