EC Number   |
Protein Variants |
Reference |
|---|
   1.14.15.22 | I243A |
complete loss of activity |
737669 |
| 1.14.15.22 | I293A |
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 5.4 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 3.4 mmol of product per min and mol of protein |
737669 |
| 1.14.15.22 | L180A |
complete loss of activity |
737669 |
| 1.14.15.22 | R193A |
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.48 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.72 mmol of product per min and mol of protein |
737669 |
| 1.14.15.22 | R193K |
complete loss of activity |
737669 |
| 1.14.15.22 | R193Q |
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 0.33 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 0.31 mmol of product per min and mol of protein |
737669 |
| 1.14.15.22 | R73A |
1alpha-hydroxyvitamin D3 hydroxylation activity at C25 30.9 mmol of product per min and mol of protein, 25-hydroxyvitamin D3 hydroxylation activity at C1alpha 20.8 mmol of product per min and mol of protein |
737669 |
| 1.14.15.22 | R73A/R84A |
variant is also capable of converting vitamin D2 to its active form, that is, 1alpha,25-dihydroxyvitamin D2, via 25-hydroxyvitamin D2, with its 1alpha-hydroxylation activity toward 25-hydroxyvitamin D2 being much lower than that toward 25-hydroxyvitamin D3. The substitutions enhance both 25- and 26-hydroxylation activity toward vitamin D2. After 25-hydroxylation of vitamin D2, further hydroxylation at C26 may occur frequently without the release of 25-hydroxyvitamin D2 from the substrate-binding pocket |
737618 |
| 1.14.15.22 | R73V/R84A |
highly active mutant, kinetic and crystallization data |
738203 |
| 1.14.15.22 | R73V/R84A |
mutant exhibits 435- and 110fold higher kcat/Km values for the 25-hydroxylation of 1alpha-hydroxyvitamin D3 and 1alpha-hydroxylation of 25-hydroxyvitamin D3, respectively, compared with the wild-type |
737668 |
