EC Number |
Protein Variants |
Reference |
---|
1.14.14.9 | H120D |
mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate |
715651 |
1.14.14.9 | H120E |
mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate |
715651 |
1.14.14.9 | H120K |
catalyzes hydroxylation with efficiency comparable to that of the wild-type enzyme, the hydroxylation rate constant for H120K is 5.7 per s and the product conversion ratio is 75%, compared to values of 16 s-1 and 90% for the wild-type enzyme |
715651 |
1.14.14.9 | H120N |
mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate |
715651 |
1.14.14.9 | H120Q |
mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate |
715651 |
1.14.14.9 | H120R |
mutant is able to catalyze hydroxylation |
715651 |
1.14.14.9 | H120Y |
mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate |
715651 |
1.14.14.9 | H155A |
drastically decreased hydroxylase activity with substrate 3-hydroxyphenylacetate |
764477 |
1.14.14.9 | H396A |
mutation of oxygenase component, decrease in hydroxylation efficiency. pKa value is 7.1 compared to 9.8 for wild-type |
744321 |
1.14.14.9 | H396N |
mutation of oxygenase component, decrease in hydroxylation efficiency. pKa value is 9.3 compared to 9.8 for wild-type |
744321 |