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EC Number
Protein Variants
Commentary
Reference
1.14.14.60
F112L
mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene, reaction of
EC 1.14.13.190
, or manoyl oxide
746484
1.14.14.60
V296L
mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene, reaction of
EC 1.14.13.190
746484
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