EC Number |
Protein Variants |
Reference |
---|
1.14.14.5 | C54A |
has little effect on FMN or FMNH2 binding, kcat/Km value decreases 6fold relative to wild-type |
702473 |
1.14.14.5 | C54S |
has little effect on FMN or FMNH2 binding, kcat/Km value increases 3fold relative to wild-type. Is able to generate the C4a-(hydro)peroxyflavin, but the rate of formation is increased 10fold relative to wild-type |
702473 |
1.14.14.5 | D111A |
kcat/Km vlaue similar to wild-type |
744319 |
1.14.14.5 | D251A/D252A/E253A |
mutation of conserved charged amino acids, 4fold decrease in kcat/Km value |
744346 |
1.14.14.5 | DELTA251-261 |
deletion of alpha-helix containing conserved charged amino acids, complete loss of activity |
744346 |
1.14.14.5 | E20A |
kcat/Km vlaue similar to wild-type |
744319 |
1.14.14.5 | F261W |
complete loss of activity |
764177 |
1.14.14.5 | R263A |
similar kinetic parameters as wild-type |
764177 |
1.14.14.5 | R263A/R271A |
similar kinetic parameters as wild-type |
764177 |
1.14.14.5 | R271A |
mutation does not alter the catalytic activity, but in the presence of reduced flavin the variant is more susceptible to proteolytic digestion compared to wild-type |
764177 |