EC Number   |
Protein Variants |
Reference |
|---|
   1.14.13.92 | A435Y |
the mutant is active only with bicyclo[3.2.0]hept-2-en-6-one |
726716 |
| 1.14.13.92 | A435Y |
the mutant shows less than 3% of wild type activity |
726716 |
| 1.14.13.92 | A442G |
the mutant shows 75% of wild type activity |
726716 |
| 1.14.13.92 | A442P |
random/saturation mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 81% conversion rate |
744548 |
| 1.14.13.92 | A442P/ L443I/S444Q |
random and site-directed mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 43% conversion rate |
744548 |
| 1.14.13.92 | A442P/ L443V/S444Q |
random and site-directed mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 45% conversion rate |
744548 |
| 1.14.13.92 | A442P/L443I |
random/saturation mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 45% conversion rate |
744548 |
| 1.14.13.92 | A442P/L443L/S444Q |
random and site-directed mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 41% conversion rate |
744548 |
| 1.14.13.92 | A442P/L443T/S444Q |
random and site-directed mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 56% conversion rate |
744548 |
| 1.14.13.92 | A442P/L443V |
random/saturation mutagenesis, the mutant is active with cyclohexanone, in contrast to the wild-type enzyme, and catalyzes its conversion to epsilon-caprolactone at 90% conversion rate |
744548 |
