EC Number   |
Protein Variants |
Reference |
|---|
   1.14.13.84 | G490A |
strictly conserved glycine, dramatic effect of mutation on binding and oxidation of NADPH |
-, 644553 |
| 1.14.13.84 | H61T |
the H61T mutant is purified as apo-enzyme and mainly exists as a dimeric species, the binding of FAD to the enzyme restores the octameric conformation |
-, 674475 |
| 1.14.13.84 | K439A |
mutant with 100fold decrease in catalytic efficiency with NADPH, mainly caused by increased Km, 4fold increased efficiency with NADH |
-, 651172 |
| 1.14.13.84 | K439F |
mutant with higher activity with NADH compared to wild-type HAPMO |
-, 651172 |
| 1.14.13.84 | K439N |
mutant with higher activity with NADH compared to wild-type HAPMO |
-, 651172 |
| 1.14.13.84 | K439P |
mutant with higher activity with NADH compared to wild-type HAPMO |
651172 |
| 1.14.13.84 | R339A |
mutant with largely decreased affinity for NADPH and decreased catalytic efficiency with NADH |
-, 651172 |
| 1.14.13.84 | R339A |
site-directed mutagenesiss, the mutant shows decreased activity and affinity to NADPH compared to the wild-type enzyme, the mutant only weakly interacts with 3-aminopyridine adenine dinucleotide phosphate |
-, 674475 |
| 1.14.13.84 | R440A |
site-directed mutagenesiss, inactive mutant, the mutant shows highly increased affinity to NADPH compared to the wild-type enzyme |
-, 674475 |
| 1.14.13.84 | R440A |
totally inactive mutant, Arg-440 is crucial for completing the catalytic cycle |
-, 651172 |
