EC Number   |
Protein Variants |
Reference |
|---|
   1.14.13.23 | A400G |
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol |
684919 |
| 1.14.13.23 | D416A |
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol |
684919 |
| 1.14.13.23 | H135P |
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform 3-aminophenol to a related substituted catechol |
684919 |
| 1.14.13.23 | K326I |
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol |
684919 |
| 1.14.13.23 | more |
screening of random mutants from a cosmid library for altered substrate specificities |
684919 |
| 1.14.13.23 | V257A |
a directed evolution study reveals that 3HB4H from Comamonas testosteroni strain GZ39 is considerably active with 4-hydroxybenzoate and that this activity increases in the V257A variant. The same variant slowly converts phenol to catechol, an activity not observed with the wild-type enzyme |
764036 |
| 1.14.13.23 | V257A |
random mutagenesis, the substitution confers the ability to the mutant enzyme to transform phenol to catechol, the mutant is also active with resorcinol, hydroquinone, p-hydroxybenzoate, 2,5-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3-chlorophenol, 4-chlorophenol, 4-chlororesorcinol, and 4-nitrophenol |
684919 |
