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EC Number
Protein Variants
Commentary
Reference
1.14.11.30
D201A
inactive
743041
1.14.11.30
D201E
the mutant shows greatly impaired activity compared to the wild type enzyme
743041
1.14.11.30
D201G
removal of the Asp ligand in the His2Asp facial triad, leads to a highly active enzyme. The variant supports anion dependent peptide hydroxylation, exogenous ligand binding to form a complete His2X facial triad is essential for O2 activation
764836
1.14.11.30
D201G
the mutant shows reduced activity compared to the wild type enzyme
743041
1.14.11.30
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FIH silencing by siRNA
724847
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