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Results 1 - 7 of 7
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4G99A (S)-lactate binds tighter to the G99A-mutant than to wild-type 440397
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4H290Q - 440424
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4H290Q the ability of L-lactate to reduce the enzyme flavin is abolished, whereas reoxidation of reduced enzyme with oxygen proceeds at a rate like that found in the wild type enzyme. Unlike the situation with wild type enzyme, where the transition state analog oxalate is bound tightly in a two-step reaction involving proton uptake from solution, the mutant enzyme binds oxalate weakly, in a single step reaction. Replacing the histidine has a significant effect on the ability of the enzyme to stabilize the flavin N(5)-sulfite adduct. Sulfite is bound at least 1000fold weaker than it is in the wild type enzyme 440400
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4K266M the rate of reduction with (S)-lactate is decreased compared with that of the wild-type enzyme, the mutant enzyme is virtually inactive 440399, 440424
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4R293K uncoupled reaction, no decarboxylation 440424
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4Y152F nearly as active as wild-type 440424
Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.4Y44F uncoupled reaction, no decarboxylation 440424
Results 1 - 7 of 7