EC Number   |
Protein Variants |
Reference |
|---|
   1.13.12.19 | A198V |
site-directed mutagenesis, the mutant produces large amounts of L-DELTA1-pyrroline-5-carboxylate but very little ethylene |
-, 742750 |
| 1.13.12.19 | A199G |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
744563 |
| 1.13.12.19 | A281V |
site-directed mutagenesis, the mutant produces low levels of products in comparison to the wild-type enzyme |
742750 |
| 1.13.12.19 | C280F |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
744563 |
| 1.13.12.19 | D191A |
site-directed mutagenesis, inactive mutant |
744563 |
| 1.13.12.19 | D191E |
the D191E variant degrades L-Arg and 2-oxoglutarate to pyrroline-5-carboxylate (again detected after reduction to proline and Fmoc derivatization) and succinate nearly stoichiometrically, with only about 5% of the cosubstrate being fragmented to ethylene |
764978 |
| 1.13.12.19 | E235D |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
744563 |
| 1.13.12.19 | E84D |
site-directed mutagenesis, the mutant does not produce ethylene |
743692 |
| 1.13.12.19 | E84Q |
site-directed mutagenesis, the mutant does not produce ethylene |
743692 |
| 1.13.12.19 | F278Y |
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme |
744563 |
