EC Number   |
Protein Variants |
Reference |
|---|
    1.13.11.15 | E266Q |
specific activity is less than 0.1% of that of the wild-type enzyme, binds 33% of the wild-type level of manganese |
-, 439624 |
| 1.13.11.15 | H155A |
specific activity is less than 0.1% of that of the wild-type enzyme, binds 0.4% of the wild-type level of manganese |
-, 439624 |
| 1.13.11.15 | H200A |
KM-value for 3,4-dihydroxyphenylacetate is 1.75fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 69fold lower than wild-type value |
-, 674920 |
| 1.13.11.15 | H200A |
Km-value for 3,4-dihydroxyphenylacetate is 6fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 33.3fold lower than wild-type value |
672032 |
| 1.13.11.15 | H200E |
KM-value for 3,4-dihydroxyphenylacetate is 1.2fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is more than 276fold lower than wild-type value, Mn content per monomer is 2fold lower than in wild-type enzyme |
-, 674920 |
| 1.13.11.15 | H200E |
Km-value for 3,4-dihydroxyphenylacetate is 75fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 11.1fold lower than wild-type value |
672032 |
| 1.13.11.15 | H200F |
Km-value for 3,4-dihydroxyphenylacetate is similar to wild-type value, kcat for 3,4-dihydroxyphenylacetate is 47.6fold lower than wild-type value |
672032 |
| 1.13.11.15 | H200F |
switch of reaction from extradiol to intradiol cleavage for substrate 2,3-dihydroxybenzoate |
655783 |
| 1.13.11.15 | H200N |
active site mutant |
713419 |
| 1.13.11.15 | H200N |
Km-value for 3,4-dihydroxyphenylacetate is 3fold lower than wild-type value, kcat for 3,4-dihydroxyphenylacetate is 3.33fold lower than wild-type value |
672032 |
