EC Number   |
Protein Variants |
Reference |
|---|
   1.11.1.B2 | D292A |
chlorinating activity is drastically reduced to approximately 1% |
348342 |
| 1.11.1.B2 | D292A |
strongly impaired in the ability to oxidize chloride but still oxidizes bromide, although inactivation occurs during turnover |
348335 |
| 1.11.1.B2 | F397H |
12% of the activity of the wild-type enzyme (chlorination of monochlorodimedone), mutant enzyme shows enhancement of bromination activity under certain conditions, inactivation of the mutant enzyme by halide especially at low pH is observed during turnover |
658904 |
| 1.11.1.B2 | H404A |
chlorinating activity is drastically reduced to approximately 1% |
348342 |
| 1.11.1.B2 | H404A |
strongly impaired in the ability to oxidize chloride but still oxidizes bromide, although inactivation occurs during turnover, reduced affinity for vanadium |
348335 |
| 1.11.1.B2 | H420F |
inactive |
725200 |
| 1.11.1.B2 | H496A |
enzyme loses the ability to bind vanadate covalently, resulting in an inactive enzyme |
348336 |
| 1.11.1.B2 | K353A |
enzyme loses the ability to effectively oxidize chloride but can still function as bromoperoxidase, no clear pH-optimum |
348336 |
| 1.11.1.B2 | P395D |
10fold increase in brominating activity at pH 8 |
674779 |
| 1.11.1.B2 | P395D/L241V/T343A |
100fold increase in brominating activity at pH 8 |
674779 |
