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EC Number	Protein Variants	Commentary	Reference
1.1.99.42	E149Q	mutant shows a showed a different pH optimum depending on the cosubstrate. With NAD+, the mutant shows very low activity with an optimum pH at 8.5 in the universal buffer. In contrast, the optimum pH is 5.5 with NADH	-, 745236
1.1.99.42	H137L	almost complete loss of activity	-, 745236
1.1.99.42	more	deletion or mutation of a deduced transmembrane segment in 4-pyridoxic acid dehydrogenase makes it an inclusion body upon expression in Escherichia coli, and the enzyme protein is not found in the cell membrane	-, 745237

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Release 2023.1 (January 2023)