EC Number |
Protein Variants |
Reference |
---|
1.1.99.37 | D100N |
lacks most of the bound cofactor NAD(H) and has lost all coenzyme NAD+-dependent MDH activity |
656101 |
1.1.99.37 | D88N |
only minor differences to wild-type in kinetics |
656101 |
1.1.99.37 | G15A |
only minor differences to wild-type in kinetics |
656101 |
1.1.99.37 | G95A |
impaired in cofactor NAD(H) binding but retains coenzyme NAD+-dependent MDH activity |
656101 |
1.1.99.37 | G95A |
low MDH activity, 10fold decrease in affinity for NAD+/NADH, insensitive to activator protein |
656101 |
1.1.99.37 | K103R |
lacks most of the bound cofactor NAD(H) and has lost all coenzyme NAD+-dependent MDH activity |
656101 |
1.1.99.37 | S97G |
impaired in cofactor NAD(H) binding but retains coenzyme NAD+-dependent MDH activity |
656101 |
1.1.99.37 | S97G |
insensitive to activator protein, but displays fully activated MDH reaction rates |
656101 |