EC Number |
Protein Variants |
Reference |
---|
1.1.3.38 | C470L |
mutant displays similar activity to the wild-type enzyme with the substrates vanillyl alcohol, chavicol aund eugenol, but no activity with linear 4-alkylphenols |
763480 |
1.1.3.38 | D170A |
3100fold decrease in turnover-number for for 4-(methoxymethyl)phenol |
655977 |
1.1.3.38 | D170A |
only 50% of the FAD is covalently bound. With vanilly alcohol, eugenol, and 4-(methoxymethyl)phenol the mutant enzyme is more than 1000fold less active than the wild-type enzyme |
654161 |
1.1.3.38 | D170A/T457E |
produces (S)-1-(4'-hydroxyphenyl)ethanol from 4-ethylphenol. The wild-type enzyme produces (R)-1-(4'-hydroxyphenyl)ethanol |
654161 |
1.1.3.38 | D170E |
2fold increase in KM-value for for 4-(methoxymethyl)phenol, 2.1fold increase in Km-value for vanillyl alcohol, 2fold decrease in KM-value for eugenol as compared to wild-type enzyme.442fold decrease in turnover-number for for 4-(methoxymethyl)phenol, 2.5fold decrease in turnover-number for vanillyl alcohol, 51.5fold decrease in turnover-number for eugenol as compared to wild-type enzyme.Redox potential of mutant enzyme, + 6 mV, is decreased compared to wild-type enzyme, 55 mV |
655977 |
1.1.3.38 | D170E |
substrate preference is similar to wild-type enzyme, as the wild-type enzyme the mutant enzyme favors the production of alkenes |
655540 |
1.1.3.38 | D170E |
with vanilly alcohol, eugenol, and 4-(methoxymethyl)phenol the mutant enzyme is 5-fold to 100fold less active than the wild-type enzyme |
654161 |
1.1.3.38 | D170N |
1550fold decrease in turnover-number for for 4-(methoxymethyl)phenol |
655977 |
1.1.3.38 | D170N |
no FAD is covalently bound. With vanilly alcohol, eugenol, and 4-(methoxymethyl)phenol the mutant enzyme is more than 1000fold less active than the wild-type enzyme |
654161 |
1.1.3.38 | D170S |
2fold decrease in KM-value for eugenol as compared to wild-type enzyme.1290fold decrease in turnover-number for for 4-(methoxymethyl)phenol, 825fold decrease in turnover-number for vanillyl alcohol, 1750fold decrease in turnover-number for eugenol as compared to wild-type enzyme. Redox potential of mutant enzyme, -91 mV, is decreased compared to wild-type enzyme, 55 mV |
655977 |