EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.82 | C207A |
strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate |
287422 |
| 1.1.1.82 | C207A/DETAEV |
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate |
287422 |
| 1.1.1.82 | C207A/E387Q |
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate |
287422 |
| 1.1.1.82 | C23A |
minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions |
287419 |
| 1.1.1.82 | C23A/C28A |
minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions |
287419 |
| 1.1.1.82 | C23A/C28A/C206A |
only marginal activity in the oxidized state, does not exhibit full activity in the crude extract |
287419 |
| 1.1.1.82 | C24S/C207A |
oxidation-reduction midpoint potential is -310 mV at pH 7.0, compared to -330 mV for the wild-type enzyme |
287425 |
| 1.1.1.82 | C24S/C29S |
oxidation-reduction midpoint potential is -280 mV at pH 7.0, compared to -330 mV for the wild-type enzyme |
287425 |
| 1.1.1.82 | C28A |
minute activity under oxidizing conditions, up to 1300fold activation under reducing conditions |
287419 |
| 1.1.1.82 | C29S |
strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate |
287422 |
