EC Number |
Protein Variants |
Reference |
---|
1.1.1.76 | F148N |
trace activity below 0.1 U/mg |
712019 |
1.1.1.76 | F212S |
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type |
761081 |
1.1.1.76 | F212W |
site-directed mutagenesis, the mutant shows highly reduced activity compared to wild-type |
761081 |
1.1.1.76 | F212Y |
site-directed mutagenesis, the kcat of the mutant is enhanced 4-8fold compared to wild-type |
761081 |
1.1.1.76 | I142Q |
trace activity below 0.1 U/mg |
712019 |
1.1.1.76 | I142Q/F148N |
trace activity below 0.1 U/mg |
712019 |
1.1.1.76 | N146A |
site-directed mutagenesis, inactive mutant |
761081 |
1.1.1.76 | N146Q |
site-directed mutagenesis, the mutant shows unaltered activity compared to wild-type |
761081 |