1.1.1.303 | more |
knockout of diacetyl reductase (DAR) gene dudA in Paenibacillus polymyxa strain ZJ-9 via homologous recombination between the genome and the previously constructed targeting vector pRN5101-L'C in a process based on homologous single-crossover. Disruption mutant Paenibacillus polymyxa ZJ-9-DELTAdudA is used for production of (R,R)-2,3-butanediol of ultra-high optical purity, and without parts of meso-2,3-butanediol, through (R,R)-butanediol dehydrogenase (EC 1.1.1.4). Paenibacillus polymyxa is a microorganism with a reported potential for the industrial production of (R,R)-2,3-butanediol. Gene dudA, the gene encoding DAR, is the key factor responsible for the formation of meso-2,3-butanediol, thus reducing the purity of (R,R)-2,3-butanediol |
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