EC Number |
Protein Variants |
Reference |
---|
1.1.1.286 | I82N |
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
-, 740021 |
1.1.1.286 | L81P |
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
-, 740021 |
1.1.1.286 | L83R |
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
740021 |
1.1.1.286 | more |
deletion of A80, inability of mutant to use 3-isopropylmalate, isocitrate is 4fold preferred over homoisocitrate |
660918 |
1.1.1.286 | more |
generation of a gene-disruption TK0280 deletion mutan strain, functional complementation by expression of the gene icdh from Thermus thermophilus strain Hb27, but not by genes hicdh and leuB from this organism |
-, 740021 |
1.1.1.286 | R85V |
complete loss of activity with citrate, retains activity with isocitrate |
-, 656127 |
1.1.1.286 | R85V |
increased ability to use 3-isopropylmalate |
660918 |
1.1.1.286 | R87T |
uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate |
660918 |
1.1.1.286 | R87V |
uses substrate homoisocitrate, but not substrates isocitrate or 3-isopropylmalate |
660918 |
1.1.1.286 | S80A |
site-directed mutagenesis, the mutant shows reduced activity and altered substrate specificity compared to the wild-type enzyme |
-, 740021 |