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Results 1 - 10 of 12 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271C109A lower Km than wild-type enzyme 389518
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271C109S active site mutant, produces GDP-6-deoxy-D-altrose as its major product (75%) besides GDP-L-fucose (25%) out of GDP-4-keto-6-deoxy-D-mannose 698467
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271C109S produces GDP-6-deoxy-D-altrose as its major product, thus C-3'' epimerization occurs first and premature reduction of the GDP-4-keto-6-deoxy-D-altrose intermediate becomes competitive with GDP-L-fucose production. Results in the appearance of a kinetic isotope effect when [3''-2H]-GDP-6-deoxy-4-ketomannose is used as a substrate. The mutant catalyzes a rapid wash-in of solvent derived deuterium into the C-5'' position of GDP-fucose in the presence of NADP+ 698467
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271H179N lower Km than wild-type enzyme 389518
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271H179Q is inactive, readily catalyzes the wash-out of deuterium from the C-3'' position of [3''-2H]-GDP-6-deoxy-4-keto-mannose 698467
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271H179Q the mutant catalyzes isotope exchange into starting material indicates that its active site has not been dramatically perturbed and it is still able to bind substrate and catalyze deprotonation events 698467
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271K140R higher Km than wild-type enzyme 389518
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271K140S higher Km than wild-type enzyme 389518
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271more effective GDP-L-fucose production in recombinant Escherichia coli by expression of GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase, method optimization at 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside, overview 677753
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.271R187A lower Km than wild-type enzyme 389518
Results 1 - 10 of 12 > >>