EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.270 | D61N |
site-directed mutagenesis, mutation in isozyme 3beta-HSD1 |
725813 |
| 1.1.1.270 | D61V |
site-directed mutagenesis, mutation in isozyme 3beta-HSD1 |
725813 |
| 1.1.1.270 | DELTA214-341 |
inactive mutant enzyme |
656795 |
| 1.1.1.270 | E192A |
site-directed mutagenesis, mutation in isozyme 3beta-HSD1 |
725813 |
| 1.1.1.270 | F303Q |
site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme |
724124 |
| 1.1.1.270 | F303Q/M304S |
site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme. The double mutation impairs the affinity and catalytic efficiency, although it did not affect the stereospecific reduction of the two 3-ketosteroids into the corresponding 3beta-hydroxysteroids |
724124 |
| 1.1.1.270 | K206A |
strain can be used to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially |
740936 |
| 1.1.1.270 | L116F |
site-directed mutagenesis |
724124 |
| 1.1.1.270 | M304S |
site-directed mutagenesis, the enzyme shows reduced 3-ketoreductase activity compared to the wild-type enzyme. The M304S mutation causes a 4fold increase in the Km value for pyridine-3-aldehyde |
724124 |
| 1.1.1.270 | more |
erg27 mutation and erg27 polymorphism in different strains and fenhexamid phenotypes, overview |
726098 |
