EC Number |
Protein Variants |
Reference |
---|
1.1.1.268 | K159A |
inactive enzyme |
-, 389507 |
1.1.1.268 | more |
comparison of steady-state kinetics and 2-butanol production arte and stereochemistry of wild-type and mutant enzymes, overview |
-, 762714 |
1.1.1.268 | R152A |
site-directed mutagenesis, the mutant shows altered substrate specificity and interaction with additives compared to wild-type enzyme |
-, 762714 |
1.1.1.268 | R152A |
the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 5192fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.3fold higher than the ratio for the wild-type enzyme |
654760 |
1.1.1.268 | R179A |
no detectable activity with 2-(2-ketopropylthio)ethanesulfonate and 2-butanone |
654760 |
1.1.1.268 | R196A |
site-directed mutagenesis, the mutant shows altered substrate specificity and interaction with additives compared to wild-type enzyme |
-, 762714 |
1.1.1.268 | R196A |
the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 1000fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 2.7fold higher than the ratio for the wild-type enzyme |
654760 |
1.1.1.268 | R203A |
site-directed mutagenesis, the mutant shows altered substrate specificity and interaction with additives compared to wild-type enzyme |
-, 762714 |
1.1.1.268 | R203A |
the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 4.3fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.6fold lower than the ratio for the wild-type enzyme |
654760 |
1.1.1.268 | R209A |
site-directed mutagenesis, the mutant shows altered substrate specificity and interaction with additives compared to wild-type enzyme |
-, 762714 |